bio rad ddseq single cell atac seq reagent box b (Bio-Rad)
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bio rad ddseq single cell atac seq reagent box b
Bio Rad Ddseq Single Cell Atac Seq Reagent Box B, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+ddseq+single+cell+atac+seq+reagent+box+b/SureCell+ATAC-Seq+Reagent+Box+A/pm41526492-293-157-158
Average 92 stars, based on 3 article reviews
Bio Rad Ddseq Single Cell Atac Seq Reagent Box B, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+ddseq+single+cell+atac+seq+reagent+box+b/SureCell+ATAC-Seq+Reagent+Box+A/pm41526492-293-157-158
Average 92 stars, based on 3 article reviews
bio rad ddseq single cell atac seq reagent box b - by Bioz Stars,
2026-10
92/100 stars
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Purification:Article Title: Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq. Article Snippet: • Chromium Next GEM Single Cell ATAC Library and Gel Bead Kit v2 (10x Genomics, cat. no. 1000390) • Chromium Next GEM Chip H Single Cell Kit (10x Genomics, cat. no.1000161) • NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544L) • VAHTS DNA Clean beads (Vazyme, cat. no. N411-01) • UltraPure DNase/RNase-free distilled water (Invitrogen, cat. no. 10977015) • Equalbit 1× dsDNA HS Assay Kit (Vazyme, cat. no. EQ121-02) • Primer oligos (all oligos were ordered from Sangon Biotech (Shanghai) with standard purification. .. Oligo sequences are listed in Supplementary Table 1) • Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, cat. no. 20034197 or 20034198) • Purified unloaded Tn5, either home-made or purchased from vendors such as EZ-Tn5 transposase (Lucigen, cat. no. TNP92110) or Tagmentase (Tn5 transposase)—unloaded (Diagenode, cat. no. C01070010) Reagents specific to mouse cortex nuclei isolation • Sucrose (Sigma-Aldrich, cat. no. V900116) • 2 M potassium chloride (Invitrogen, cat. no. AM9640G) • 1 M magnesium chloride (Sigma-Aldrich, cat. no. 63069-100ML) • Protease Inhibitor Cocktail (Roche, cat. no. 04693132001) • RNasin (Promega, cat. no. N2111) Reagents specific to the plate workflow • 4′,6-Diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific, cat. no.62248) • 10% (wt/vol) SDS(Sigma-Aldrich, cat. no. L4509) • DNA clean and concentrator-5 (Zymo, cat. no. D4014) • Buffer PB (Qiagen, cat. no. 19066) Reagents specific to the Bio-Rad workflow • Bio-Rad ddSEQ M cartridges (Bio-Rad, cat. no. 12009359) • Bio-Rad ddSEQ Single-Cell ATAC-Seq Reagent Box A (Bio-Rad, cat. no. 12009358) Isolation:Article Title: Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq. Article Snippet: • Chromium Next GEM Single Cell ATAC Library and Gel Bead Kit v2 (10x Genomics, cat. no. 1000390) • Chromium Next GEM Chip H Single Cell Kit (10x Genomics, cat. no.1000161) • NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544L) • VAHTS DNA Clean beads (Vazyme, cat. no. N411-01) • UltraPure DNase/RNase-free distilled water (Invitrogen, cat. no. 10977015) • Equalbit 1× dsDNA HS Assay Kit (Vazyme, cat. no. EQ121-02) • Primer oligos (all oligos were ordered from Sangon Biotech (Shanghai) with standard purification. .. Oligo sequences are listed in Supplementary Table 1) • Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, cat. no. 20034197 or 20034198) • Purified unloaded Tn5, either home-made or purchased from vendors such as EZ-Tn5 transposase (Lucigen, cat. no. TNP92110) or Tagmentase (Tn5 transposase)—unloaded (Diagenode, cat. no. C01070010) Reagents specific to mouse cortex nuclei isolation • Sucrose (Sigma-Aldrich, cat. no. V900116) • 2 M potassium chloride (Invitrogen, cat. no. AM9640G) • 1 M magnesium chloride (Sigma-Aldrich, cat. no. 63069-100ML) • Protease Inhibitor Cocktail (Roche, cat. no. 04693132001) • RNasin (Promega, cat. no. N2111) Reagents specific to the plate workflow • 4′,6-Diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific, cat. no.62248) • 10% (wt/vol) SDS(Sigma-Aldrich, cat. no. L4509) • DNA clean and concentrator-5 (Zymo, cat. no. D4014) • Buffer PB (Qiagen, cat. no. 19066) Reagents specific to the Bio-Rad workflow • Bio-Rad ddSEQ M cartridges (Bio-Rad, cat. no. 12009359) • Bio-Rad ddSEQ Single-Cell ATAC-Seq Reagent Box A (Bio-Rad, cat. no. 12009358) Protease Inhibitor:Article Title: Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq. Article Snippet: • Chromium Next GEM Single Cell ATAC Library and Gel Bead Kit v2 (10x Genomics, cat. no. 1000390) • Chromium Next GEM Chip H Single Cell Kit (10x Genomics, cat. no.1000161) • NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544L) • VAHTS DNA Clean beads (Vazyme, cat. no. N411-01) • UltraPure DNase/RNase-free distilled water (Invitrogen, cat. no. 10977015) • Equalbit 1× dsDNA HS Assay Kit (Vazyme, cat. no. EQ121-02) • Primer oligos (all oligos were ordered from Sangon Biotech (Shanghai) with standard purification. .. Oligo sequences are listed in Supplementary Table 1) • Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, cat. no. 20034197 or 20034198) • Purified unloaded Tn5, either home-made or purchased from vendors such as EZ-Tn5 transposase (Lucigen, cat. no. TNP92110) or Tagmentase (Tn5 transposase)—unloaded (Diagenode, cat. no. C01070010) Reagents specific to mouse cortex nuclei isolation • Sucrose (Sigma-Aldrich, cat. no. V900116) • 2 M potassium chloride (Invitrogen, cat. no. AM9640G) • 1 M magnesium chloride (Sigma-Aldrich, cat. no. 63069-100ML) • Protease Inhibitor Cocktail (Roche, cat. no. 04693132001) • RNasin (Promega, cat. no. N2111) Reagents specific to the plate workflow • 4′,6-Diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific, cat. no.62248) • 10% (wt/vol) SDS(Sigma-Aldrich, cat. no. L4509) • DNA clean and concentrator-5 (Zymo, cat. no. D4014) • Buffer PB (Qiagen, cat. no. 19066) Reagents specific to the Bio-Rad workflow • Bio-Rad ddSEQ M cartridges (Bio-Rad, cat. no. 12009359) • Bio-Rad ddSEQ Single-Cell ATAC-Seq Reagent Box A (Bio-Rad, cat. no. 12009358) Single Cell:Article Title: Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq. Article Snippet: • Chromium Next GEM Single Cell ATAC Library and Gel Bead Kit v2 (10x Genomics, cat. no. 1000390) • Chromium Next GEM Chip H Single Cell Kit (10x Genomics, cat. no.1000161) • NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544L) • VAHTS DNA Clean beads (Vazyme, cat. no. N411-01) • UltraPure DNase/RNase-free distilled water (Invitrogen, cat. no. 10977015) • Equalbit 1× dsDNA HS Assay Kit (Vazyme, cat. no. EQ121-02) • Primer oligos (all oligos were ordered from Sangon Biotech (Shanghai) with standard purification. .. Oligo sequences are listed in Supplementary Table 1) • Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, cat. no. 20034197 or 20034198) • Purified unloaded Tn5, either home-made or purchased from vendors such as EZ-Tn5 transposase (Lucigen, cat. no. TNP92110) or Tagmentase (Tn5 transposase)—unloaded (Diagenode, cat. no. C01070010) Reagents specific to mouse cortex nuclei isolation • Sucrose (Sigma-Aldrich, cat. no. V900116) • 2 M potassium chloride (Invitrogen, cat. no. AM9640G) • 1 M magnesium chloride (Sigma-Aldrich, cat. no. 63069-100ML) • Protease Inhibitor Cocktail (Roche, cat. no. 04693132001) • RNasin (Promega, cat. no. N2111) Reagents specific to the plate workflow • 4′,6-Diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific, cat. no.62248) • 10% (wt/vol) SDS(Sigma-Aldrich, cat. no. L4509) • DNA clean and concentrator-5 (Zymo, cat. no. D4014) • Buffer PB (Qiagen, cat. no. 19066) Reagents specific to the Bio-Rad workflow • Bio-Rad ddSEQ M cartridges (Bio-Rad, cat. no. 12009359) • Bio-Rad ddSEQ Single-Cell ATAC-Seq Reagent Box A (Bio-Rad, cat. no. 12009358) |